Primary Structure Determination Self Test:
Question #1: 
A polypeptide chain is digested with chymotrypsin resulting in the following fragments:
                LLVATSS
                GNADMSCGTGY
                GVHAAIMLIQF   
Another sample of the same polypeptide chain is then treated with cyanogen bromide resulting in the following fragments:
                SCGTGYLLVATSS
                GVHAAIM
                LIQFGNADM
What is the primary structure of the polypeptide chain?

GVHAAIMLIQFLLVATSSGNADMSCGTGY
GNADMSCGTGYGVHAAIMLIQFLLVATSS
GVHAAIMLIQFGNADMSCGTGYLLVATSS
SCGTGYLLVATSSGVHAAIMLIQFGNADM
LIQFGNADMGVHAAIMSCGTGYLLVATSS

 

Question #2: 
A polypeptide chain is digested with pepsin resulting in the following fragments:
                YERIHGSD
                GALDEET
                YJITJ
                FAERJALSJDI
Another sample of the same polypeptide chain is digested with Submaxillarus protease resulting in the following fragments:
                GALDEETYER
                JALSJDIYJITJ
                IHGSDFAER
What is the primary structure of the polypeptide chain?

GALDEETYERIHGSDFAERJALSJDIYJITJ
IHGSDFAERGALDEETYERJALSJDIYJITJ
JALSJDIYJITJIHGSDFAERGALDEETYER
GALDEETYERIHGSDYJITJFAERJALSJDI
FAERJALSJDIYJITJYERIHGSDGALDEET


Question #3: 
A polypeptide chain is digested with trypsin resulting in the following fragments:
                 YANDBIOCHEMISTR
                 YSALLY
                 LOVESCHEMISTR
Another sample of the same polypeptide chain is digested with Staphylococcus aureus resulting in the following fragments:
                MISTRYANDBIOCHE
                LOVE
                MISTRYSALLY
                SCHE
What is the primary structure of the polypeptide chain?

SALLYLOVESCHEMISTRYANDBIOCHEMISTRY
LOVESCHEMISTRYANDBIOCHEMISTRYSALLY
CHEMISTRYANDBIOCHEMISTRYSALLYLOVES
ANDBIOCHEMISTRYSALLYLOVESCHEMISTRY
BIOCHEMISTRYSALLYLOVESCHEMISTRYAND

 

Question #4: 
A pentapeptide is treated with FDNB and then hydrolyzed with 6M HCl.  The 2,4-dinitrophenyl derivative of alanine was detected.  When the pentapeptide is digested with pepsin, two fragments are formed.  One fragment contained one alanine and one valine.  The other fragment contained two serines and one phenylalanine.  What is the structure of the pentapeptide?

Ser-Ser-Phe-Val-Ala
Ala-Val-Phe-Ser-Ser
Phe-Ser-Ser-Val-Ala
Ala-Val-Ser-Ser-Phe
Val-Ala-Phe-Ser-Ser


Question #5: 
A polypeptide chain is digested with pepsin resulting in the following fragments:
                 YMOREDRHORNBUCKLE
                 WANTS
                 YOUTOSTUD
Another sample of the same polypeptide chain is digested with trypsin resulting in the following fragments:
                EDR
                NBUCK
                WANTSYOUTOSTUDYMOR
                HOR
                LE
What is the primary structure of the polypeptide chain?

DRHORNBUCKLEWANTSYOUTOSTUDYMORE
YMOREDRHORNBUCKLEYOUTOSTUDWANTS
YOUTOSTUDYMOREDRHORNBUCKLEWANTS
HORNBUCKLEWANTSYOUTOSTUDYMOREDR
WANTSYOUTOSTUDYMOREDRHORNBUCKLE