|
DATE
|
TOPICS
|
Reading
for Lab
|
Handouts
(stuff to download and print) |
|
Jan 10
|
 | No Lab
|
|
|
|
| Jan 12 |
| Introduction to the lab (Mandatory)
|
|
Lab Safety Pages 2-5 |
Laboratory
Safety Objectives Safety
Power Point
List of Microorganisms
used in Lab |
| Jan 17 |
No Class - Martin Luther King Jr. Day |
|
|
| Jan 19, 24 |
Lab 1
| What is asepsis? |
| Microbes Everywhere |
| Media types |
| Making media and pouring plate |
| Aseptic
technique |
| Growth of bacteria in liquids, deeps and slants |
|
Handout 1, and
pages 6-7, 14-19, and 23-26 Handout 1 and pages 28-41 and 62 |
Handout 1 |
| Jan 26, 31 |
Lab 2
| Isolating Bacteria/selection media |
| Streak plating |
| Colonial morphology or growth of bacteria and
fungi on plates |
|
Handout 2 and pages 42-47 and pages 60-61 |
Handout
2 |
|
Feb 2, 7 and 9 |
Lab 3
| Brightfield microscopy |
| Phase contrast Microscopy
|
| Bacterial cellular morphology |
| Identifying eukaryotic microbes under the microscope |
|
Handout 3 and pages 74-86. |
Handout 3
Slides to Know
PowerPoint |
| Feb 14 |
Lab 4
|
Practice Streak Plating (lab 3
above) |
|
Smear Preparation |
|
Simple Stain |
|
Handout 4 and pages 87-93 |
Handout 4
Power
Point |
| Feb 16, 21, and 23 |
Lab 5
| Gram Stain |
| Acid Fast Stain |
| Spore Stain |
|
Handout 5 Pages 94-104 and 112-115 |
Gram Stain Handout
Acid
Stain
Spore Stain
Handout 5 |
| Feb 28 |
Practical 1 covering labs
1-5 and start Skills Test |
|
|
| March 2 |
Skills Test: covers media
pouring, aseptic technique, streak plating, smear making, staining,
microscopy |
|
|
|
March 4
Midterm Drop Date: Last day to
drop the course and receive a W
|
| March 7, 9 |
No Classes - Spring Break |
|
|
| March 14, 16 |
Lab 6
| Population Counts |
|
Handout 6 pages 56-59 and 183-197
|
Handout
6
Report
Sheet
Practice Problems
Enumeration
PowerPoint |
| March 21, 23 |
Lab 7
| Temperature Effects on Growth
|
|
Oxygen Requirements |
|
Handouts for 7 |
Temperature
Lab
Oxygen Requirements Lab |
| March 28, 30 |
Lab 8
| Control of Microbial Growth
 | UV light Effects on Growth
|
| Evaluation of antibiotics
|
|
|
Handouts for 8 and pages 206-212 |
Antibiotic Lab
UV Light Lab |
| April 4, 6 |
Lab 9
| Control of Microbial Growth
| Optimal Growth Conditions |
| Lethal Effects of Heat |
|
|
|
Optimal Growth Conditions Lab
Lab
Report/Presentation
Lethal Effects of Heat |
|
April 11, 13 |
Lab 10 Case Study |
Case Study |
Case Study
Media PowerPoint
Media Study Guide |
| April 18, 20 |
Lab 11
Investigating Sulfa Drugs |
Handouts posted |
Lab
Protocol
Derivative Sheet
Possible Sulfa Drugs to
choose from
|
| April
25 |
Practical 2
covering labs 6-10
|
|
|
| April 27 and May 2 |
No Lab |
|
|