BIOL3250L Tentative Course Schedule

DATE

TOPICS

Reading for Lab

Handouts (stuff to download and print)

Aug 17-20

  • No Lab (Aug 17-18)
  • Introduction to the lab (Mandatory)
Lab Safety Guidelines in Manual (Pages 2 - 5) Laboratory Safety Objectives

List of Microorganisms used in Lab

       
Aug 24 - 27

Lab 1

  • Microbes Everywhere
  • Media types
  • Making media and pouring plates
Pages 6-7, 14-19, and 23-26 Handout 1
Aug 31, Sept 1-3

Lab 1 (continued)

  • Aseptic Technique
  • Growth of bacteria in liquids, deeps and slants
Pages 28-41 and 62 Handout 1 (see above)
Sept 7-8

LABOR DAY - NO CLASSES

   
Sept 9-10, 14-15

Lab 2

  • Isolating Bacteria/selection media
  • Streak plating 
  • Colonial morphology or growth of bacteria and fungi on plates

Pages 42-47 and pages 60-61 Handout 2
Sept 14-17

Lab 3

  • Brightfield microscopy
  • Phase contrast microscopy
  • Bacterial cellular morophology
  • Identifying eukaryotic microbes under the microscope
  • Practice streak plating

Pages 74-86 Handout 3

Slides to Know

Sept 21-22

Lab 4

  • Smear preparation
  • Simple stain
Pages 87-93 Handout 4

PowerPoint

Sept 23-24, and Sept 28 - Oct 1

Lab 5

  • Gram Stain
  • Acid Fast Stain
  • Spore Stain
Pages 94-104 and 112-115 Handout 5
Sept 30 - Oct 1 Review for Practical 1    

Oct 5 - 6

Practical 1 covering labs 1 - 5 and start Skills Test  

 

Oct 7 -8 Skills Test:  covers media pouring, aseptic technique, streak plating, smear making, staining, microscopy    
Oct 9

Midterm Drop Date:  Last day to drop the course and receive a W

Oct 12 - 15

Lab 6

  • Population Counts
Pages 56-59 and 183-197 Handout 6

Report Sheet

Practice Problems

Enumeration PowerPoint

Oct 19 -22

Lab 7

  • Temperature Effects on Growth
  • Optimal Growth Conditions
  • Oxygen Requirements

  Temperature Handout

Optimal Growth Conditions

Oxygen Requirements

Lab Report/Presentation

Oct 26 - 29

Lab 8

  • Control of Microbial Growth
    • UV light Effects on Growth
    • Evaluation of antibiotics

Pages 206-212 Antibiotic Lab

UV Light Lab

Nov 2 - 5

Lab 9

  • Antiseptic and Disinfectant Susceptibility Lab

 

Antiseptic and Disinfectant Lab
Nov 9 - 12  Lab 10
  • Case Study

  Case Study Handout

Media Study Guide

Media PowerPoint

Willams PowerPoint

Nov 16 - 19 Wrap up and review    
Nov 23 - 24

Practical 2 covering labs 5-10