BIOL4202 Lab
LAB SCHEDULE SPRING 2008 - Subject to change
PART 1 Complete
PART 2 Complete
PART 3 Gene Detection and Expression ( Southern Blotting/ Protein Isolation/Western Blotting, Size Exclusion, Bacterial Transformation, Protein Purification)- March and April
PART 4 Specialized Techniques (ELISA, Forensic Analysis, Genetically Engineering Organisms, Tissue Printing)-April
PART 3 Gene Detection and Expression
| WEEK | DAY | LECTURE TOPIC | LABORATORY EXERCISES | ASSESSMENT |
| 1
|
March 25-27 | Genes and Genetics Chapter 3- Powerpoints Overview of Molecular Biology Chapter 4- Powerpoints
|
Southern Blotting Protein Determination
|
Study Activity #16 in Textbook- Due 3/27/08
Southern Blotting
|
| 2 | April 1 - April 3 | Introduction to Recombinant DNA technology Chapter 5 - Power points
|
Southern Blotting Introduction to SDS-PAGE
|
Southern Blotting mini-lab due- April 3rd Assign Case Study #2 |
| 3 | April 8 - 10 | Chapter 5 Classroom Activities Societal Issues |
SDS PAGE
Western Blotting |
Quiz on Southern Blotting - April 8 Pre-lab on SDS - Due April 8th Assign Lab Report #3 |
| 4 | April 15 - 17 | Chapter 5 - cloning
Preparing a Research Proposal - Handout Societal Issues: Bioethics. Genetically Modified Organisms |
Complete SDS-PAGE Bacterial Transformation
Column Chromatography |
Quiz on SDS- Page - April 15 Case Study #2 Due April 17th Pre-Lab on Bacterial - Due April 15th
|
| 3 | April 22 - 24 |
Test 3 - April 22nd Chapter 3,4, and 5, Protein Determination, Southern Blotting, SDS-PAGE, Western
New Material for Final Exam- Powerpoints |
Specialty labs: Complete pGLO and Green Protein Genetically Modified Organisms Forensic Analysis ELISA
|
Lab Report #3 Due April 22nd 1-2 page Research Proposal- Due 5/01//08- LAST ASSIGNMENT for BIOTECH
Notebooks Due 4/24/08
|
FINAL EXAM INFORMATION: Comprehensive (Lecture and Lab Activities), May 1st 3pm - 5pm