LOCUS LPZ-008.CR 237 BP DNA ENTERED 05/20/1997 Orientation in vector: unknown KEYWORD Loblolly pine, embryogenesis, zygotic embryo SOURCE Cloned from differential display band ORGANISM Pinus taeda COMMENT This sequence was entered by Nanfei Xu. A band named Z9.3C4-2 was generated from zygotic embryos of loblolly pine using T12MC and AP-4 primers in differential display. The band was present at stages 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9 and 9.10, and was most abundant at stage 9.10. The band was cloned using EcoR5 digested PKS vector. One clone was identified and sequenced using M13 reverse primer. The 116-236 of this sequence is identitical to the 209-329 bp region of LPZ006. GeneBank search showed that this sequence has 77% identities with a Lily mRNA from flowering buds. .. 1 GGTGCGATCC AAAGAACACA AGATGGAGTT ACCACAATGG AGGATCTTGG CCAGTGCTTT 61 TGTGGCTATT CACTGCAGCC TGTATTAAAA CAGGAAGGCC GCAAATGGCC AGAAGGGCCA 121 TTGAACTTGC TGAGAGCAGA CTATCTAAGG ATGGCTGGCC TGAATATTAT GATGGGAAGC 181 TTGGACGATA TATTGGAAAG CAGTCTCGAA AGTGGCAAAC CTGGTCAGTT GCTGGAT