BIOL3250L Course Schedule*

DATE

TOPICS

Reading for Lab

Handouts (stuff to download and print)

Jan. 13
Jan. 15

bulletNo Lab
bulletIntroduction to the lab (Mandatory)
bulletWhat is asepsis?
Lab Safety Pages 2-5 Laboratory Safety Objectives

List of Microorganisms used in Lab

Jan. 17-19 Dr. Martin Luther King Holiday:   No Class    
Jan. 20 and 22

Lab 1

bulletMicrobes Everywhere
bulletMedia types
bulletMaking media and pouring plates
Handout 1, and
pages 6-7, 14-19, and 23-26
Handout 1

 
Jan. 22 and 27

Lab 1 continued

bulletAseptic technique
bulletGrowth of bacteria in liquids, deeps and slants
Handout 1 and pages 28-41 and 62 Handout 1 (above)
Jan. 27 and 29

Lab 2

bulletIsolating Bacteria/selection media
bulletStreak plating 
bulletColonial morphology or growth of bacteria and fungi on plates
Handout 2 and pages 42-47 and pages 60-61 Handout 2
Feb. 3, 5 and 10, 12

Lab 3
bulletBrightfield microscopy
bulletPhase contrast Microscopy 
bulletBacterial cellular morphology
bulletIdentifying eukaryotic microbes under the microscope
bulletPractice steak plating

Handout 3 and pages 74-86. Handout 3

Slides to Know

PowerPoint

Feb. 17

Lab 4
bullet

Smear Preparation

bullet

Simple Stain

Handout 4 and pages 87-93

Handout 4

Power Point

Feb. 19 and 24

Lab 5

bulletGram Stain
bulletAcid Fast Stain
bulletSpore Stain
Handout 5 Pages 94-104 and 112-115 Gram Stain Handout

Acid Stain

Spore Stain

Handout 5

Feb. 24 Review    
 

Feb. 26

Practical 1 covering labs 1-5 and start Skills Test  

 

March 3 Skills Test:  covers media pouring, aseptic technique, streak plating, smear making, staining, microscopy    
March 6

Midterm Drop Date:  Last day to drop the course and receive a W

March 5

Lab Canceled

   
Spring Break March 7-15:  No Class
March 17, 19

Lab 6

bulletPopulation Counts

 

Handout 6

pages 56-59 and 183-197

 
Handout 6

Report Sheet

Practice Problems

Enumeration PowerPoint 

March 24, 26

Lab 7
bulletTemperature Effects on Growth
bulletOptimal Growth Conditions
bullet

Oxygen Requirements

Handouts for 7 Temperature Lab

Optimal Growth Conditions Lab

Oxygen Requirements Lab

Lab Report/Presentation

 

March 31 and April 2

Lab 8
bulletControl of Microbial Growth
bulletUV light Effects on Growth
bulletEvaluation of antibiotics
bulletBacteriocins and microbial competition

Handouts for 8 and pages 206-212 Antibiotic Lab

UV Light Lab

New Lab coming

 

April 7 and 9

Lab 9
Discovering Antibiotics

Given in Class Antibiotic Lab

Report Sheet

 April 14 and 16

Lab 10
bullet

Case Study

Case Study

Case Study

Media PowerPoint

April 21

Finish Case Study

   
April 23 Wrap up day and review    
April 28

Practical 2 covering labs 6-10

   
April 30 No Lab    

*This is a tentative schedule.  It may change so check it periodically.