BIOL3250L Course Schedule*

DATE

TOPICS

Reading for Lab

Handouts (stuff to download and print)

Aug. 21
Aug. 23

bulletNo Lab
bulletIntroduction to the lab (Mandatory)
Lab Safety Guidelines in Manual Laboratory Safety Objectives
       
Aug. 28 and 30

Lab 1
bulletBrightfield microscopy
bulletPhase contrast Microscopy 
bulletBacterial and fungal cellular and colonial morphology
bulletProtozoa, Algae and Helminth morphology

Pages 1-19 Lab Session 1 Objectives 

Lab 1 Instructions

PowerPoint for Lab 1

Sept. 6, 11, 13 and 18

Lab 2

bulletMicrobes Everywhere
bulletMaking media, pouring plates, aseptic technique
bulletMotility
bulletIsolating Bacteria/selection media
bulletStreak plating 
pages 49-63, 65-66 and 73-76 Lab Session 2 Objectives

Lab 2 Instructions

Sept. 20

Lab 3
bullet

Smear Preparation

bullet

Simple Stain

bullet

Continue Streak plating

Pages 21-24 Lab Session 3 and 4 Objectives 

Lab 3and 4 Instructions

Power Point

Sept. 20, 25, 27 and Oct. 2

Lab 4

bulletGram Stain
bulletAcid Fast Stain
bulletSpore Stain
bulletCapsule Stain
Pages 27-44 See above for objectives and instructions.
 

Oct. 4

Practical 1 covering labs 1-4 and start Skills Test  

 

Oct. 9 Skills Test:  covers media pouring, aseptic technique, streak plating, smear making, staining, microscopy    
October 12

Midterm Drop Date:  Last day to drop the course and receive a W

October 11:  TBA

Oct. 16 and 18

Lab 5

bulletPopulation Counts
Lab Protocol

Report Sheet

Objectives 5

Practice Problems

Enumeration PowerPoint

Oct. 23 and 25

Lab 6
bulletTemperature Effects on Growth
bulletOsmotic Pressure effects on Growth
bullet

Oxygen Requirements

Pages 77-92 and 105-110

Temperature Handouts

Lab Session 6 Objectives 

Temperature Handout

 

Oct. 30 and Nov. 1

Lab 7
bulletControl of Microbial Growth
bulletUV light Effects on Growth
bulletEvaluation of antibiotics
bulletLethal Effects of heat

Pages 99 - 113

UV handout

Lab Session 7 Objectives 

UV Handout

Nov. 6 and 8

Lab 8
bullet

Microbes and Me
bullet

Cavity Creeps

bullet

Exploring your Flora

bullet

The Use Dilution Method

Handout Protocol 1

Handout protocol 2

 

Lab Session Objectives 8
Nov. 13, 15, and 20th  Lab 9
bullet

Benzocaine derivatives and UV blockers

Power Point Lab Session 9 objectives coming soon
Nov. 22 Wrap up day and review
bullet

Wrap up the Osmotic Tolerance Lab and Discuss

bullet

Wrap up the Kirby Baur Lab and discuss

bullet

Presentations from the Use Dilution Lab and discussion

   
Nov. 27

Practical 2 covering labs 5-10

   

*This is a tentative schedule.  It may change so check it periodically.