BIOL3250L Course Schedule*

DATE

TOPICS

Reading for Lab

Handouts (stuff to download and print)

Aug. 19
Aug. 21

bulletNo Lab
bulletIntroduction to the lab (Mandatory)
bulletWhat is asepsis?
Lab Safety Pages 2-5 Laboratory Safety Objectives

List of Microorganisms used in Lab

Sept. 2 No Class    
Aug. 26, 28

Lab 1

bulletMicrobes Everywhere
bulletMedia types
bulletMaking media and pouring plates
Handout 1, and
pages 6-7, 14-19, and 23-26
Handout 1

 
August 28 and Sept. 4

Lab 1 continued

bulletAseptic technique
bulletGrowth of bacteria in liquids, deeps and slants
Handout 1 and pages 28-41 and 62 Handout 1 (above)
Sept. 4, 9,

Lab 2

bulletIsolating Bacteria/selection media
bulletStreak plating 
bulletColonial morphology or growth of bacteria and fungi on plates
Handout 2 and pages 42-47 and pages 60-61 Handout 2
Sept. 11, 16 and 18

Lab 3
bulletBrightfield microscopy
bulletPhase contrast Microscopy 
bulletBacterial cellular morphology
bulletIdentifying eukaryotic microbes under the microscope
bulletPractice steak plating

Handout 3 and pages 74-86. Handout 3

Slides to Know

PowerPoint

Sept. 23

Lab 4
bullet

Smear Preparation

bullet

Simple Stain

Handout 4 and pages 87-93

Handout 4

Power Point

Sept.25, 30 and Oct. 2

Lab 5

bulletGram Stain
bulletAcid Fast Stain
bulletSpore Stain
Handout 5 Pages 94-104 and 112-115 Several Handouts will be given in class for reading.

Handout 5

 

Oct. 7

Practical 1 covering labs 1-5 and start Skills Test  

 

Oct. 9 Skills Test:  covers media pouring, aseptic technique, streak plating, smear making, staining, microscopy    
October 10

Midterm Drop Date:  Last day to drop the course and receive a W

October 11:  TBA

Oct. 14

Lab 6
Phenanthene Bioremediation Lab

Creating the Microcosms

Handout 6 Handout 6

Power Point

Lab Report Grading Rubric

Oct. 16 and 21

Lab 6
Phenanthene Bioremediation Lab

The plating experiment

   
Oct. 28 and 30

Lab 7

bulletPopulation Counts
Handout 7

pages 56-59 and 183-197

 

Handout 7

Report Sheet

Practice Problems

Enumeration PowerPoint

Nov. 4, 6

Lab 8
bulletTemperature Effects on Growth
bulletOsmotic Pressure effects on Growth
bullet

Oxygen Requirements

Handout 8 Temperature Lab

Osmotic Tolerance Lab

Oxygen Requirements Lab

Nov. 11, 13,

Lab 9
bulletControl of Microbial Growth
bulletUV light Effects on Growth
bulletEvaluation of antibiotics

Handout 9 and pages 206-212 Antibiotic Lab

UV Light Lab

 18 and 20

Lab 10
bullet

The Epidemic

Handout 10

Handout 10

Data Sheet

Nov. 25 Wrap up day and review    
Dec. 2

Practical 2 covering labs 6-10

   

*This is a tentative schedule.  It may change so check it periodically.