Determining properties of SA

                                                                                    April 8:    Swab nostrils

Inoculate m-Staph broth (selective media)

Only salt tolerant Staph will grow

April 13 Background to project                                            m-Staph broth

      (Staph aureus)         (Staph epidermidis)

SA (+)                         SE (-)

Mannitol Salt               Mannitol Salt                                       Mannitol Salt

(selective and differential)   Selects for salt tolerant Staph

Differentiates mannitol   fermentors from non fermentors

April 15:

1. Streak BAP                   Streak BAP                       Streak BAP (look for hemolysis)

+ Novobiocin                  + Novobiocin                      + Novobiocin (antibiotic

sensitivity of SA and SE but

not Staph saprophyticus)

2. Prepare Slant                 Prepare Slant                     Prepare Slant

April 20

Gram stain                               Gram stain                   Gram stain

Coagulase test                         Coagulase test             Coagulase test (+ for SA)

Staphyloside                            Staphyloside                Staphyloside (+ for SA)

April 22:

Analyze results, take final quiz and review for practical