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BIOL3250L Tentative Course Schedule - Spring 2010

DATE	TOPICS	Reading for Lab	Handouts (stuff to download, print or complete)
Jan. 14	No Lab (January 12) Introduction to the lab (Mandatory) - Jan. 14	Lab Safety Guidelines in Manual (Pages 2 - 5)	Laboratory Safety Objectives List of Microorganisms used in Lab

Jan. 19 - 21	Lab 1 Microbes Everywhere Media types Making media and pouring plates	Pages 6-7, 14-19, and 23-26	Handout 1 Picture of environmental microbes Lab 1 prelab - complete questions on Georgia View Due Jan. 19 at 12:35 PM
Jan. 26 - 28	Lab 1 (continued) Aseptic Technique Growth of bacteria in liquids, deeps and slants	Pages 28-41 and 62	Handout 1 (see above) Aseptic technique demonstration Media types Lab 1 - unknown identification
Feb. 2-4	Lab 2 Isolating Bacteria/selection media Streak plating Colonial morphology or growth of bacteria and fungi on plates	Pages 42-47 and pages 60-61	Handout 2 Streaking a plate demonstration Lab 2 prelab - complete questions on Georgia View Due on Feb. 2 at 12:35 PM Fungi morphology
Feb. 9 - 11 and 16 - 18	Lab 3 Brightfield microscopy Phase contrast microscopy Bacterial cellular morophology Identifying eukaryotic microbes under the microscope Practice streak plating	Pages 74-86	Handout 3 Slides to Know Lab 3 prelab - complete questions on Georgia View Due on Feb. 9 at 12:35 PM Lab 3 - unknown identification
Feb. 23	Lab 4 Smear preparation Simple stain	Pages 87-93	Handout 4 Lab 4 prelab - complete questions on Georgia View Due on Feb. 23 at 12:35 PM Lab 4 and 5 - unknown identification
Feb. 25 and Mar. 2	Lab 5 Gram Stain Acid Fast Stain Spore Stain start Skills Test - streak plate Description of skills test	Pages 94-104 and 112-115	Handout 5 Lab 5 prelab - complete questions on Georgia View Due on Feb. 25
Mar. 4	Practical 1 covering labs 1 - 5 and part of Skills Test (smear making and staining)
	Mar. 5 - Midterm drop date. Last day to drop course and receive a grade of W.
	Mar. 6 - 13: Spring Break, No class
Mar. 16	Finish Skills Test: covers media pouring and microscopy
Mar.18 - Field trip to the CDC - leave campus at 12:00, tour is at 1:00 Directions
Mar. 23 - 25	Lab 6 Population Counts	Pages 56-59 and 183-197	Handout 6 Report Sheet Lab 6 Power Point How to use the spectrophotometer Sample problems Lab 6 prelab - complete questions on Georgia View Due on Mar. 23
Mar. 30 - Apr. 1	Lab 7 Temperature Effects on Growth Optimal Growth Conditions Oxygen Requirements		Temperature Handout Growth lab Oxygen lab PowerPoint 1 PowerPoint 2 Lab 7 prelab - complete questions on Georgia View Due on Mar. 30 Lab 7 - unknown identification
Apr. 6 - 8	Lab 8 Control of Microbial Growth UV light Effects on Growth Evaluation of antibiotics	Pages 206-212	UV lab Antibiotic lab Lab 8 prelab - complete questions on Georgia View Due on Apr. 6 Lab 8 - unknown identification
Apr. 13 - 15	Lab 9 Biochemical Tests		Book review for practical Jeopardy review for Practical 2 Biochemical tests protocol Lab9 Biochemical Tests PowerPoint Media Study guide Update to unknown project No Lab 9 prelab - instead EVERYONE must complete the media study guide after we complete the lab
Apr. 20 - 22	Lab 10 Immunology		Media Study Guide due on Apr. 20 Lab report on unknown due on Apr. 22 Antigen/antibody complexes Uses for antibodies Immunology objectives Ouchterlony protocol Radial Immunodiffusion protocol Lab 10 prelab - complete questions on Georgia View Due on Apr. 20
Apr. 27	Wrap up and review (class will end at 1:40 pm)
Apr. 29	Practical 2 covering labs 5-10