Microbiology 2250 Lab Schedule

 

 

Date Lab Exercise Read Before Lab Objectives and Other Things to Download Before Lab

1/12

No Lab Today    

1/14

Introduction to the lab (NOT OPTIONAL)
(If you don't show up for this you will be DROPPED from the Lab)
Laboratory Safety Guidelines Lab Safety Powerpoint
1/19

(No class MLK day)

1/21, 1/26 and 1/28

Lab 1 

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Brightfield microscopy and cellular morphology.

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Microbes in the Environment and Colonial Morphology

 
 

 

Pictures of organisms

Pictures of bacteria

Pictures of environmental microbes

Lab 1 objectives

Lab 1 power point

Microscopy Handout

Microscopy Lab Report

Microbes Handout

2/2, 2/4 and 2/9

(2/9 finish lab 2)

Lab 2

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Aseptic Technique

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Bacterial Isolation by Streak Plate

 
  Lab 2 objectives


Lab 2 background and protocol

Aseptic technique powerpoint

Media

Streaking a plate

 

2/9, 2/11

(2/9 start lab 3)

(2/11 start lab 4)

Lab 3

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Smear Preparation

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Simple Stain

 
 

 


Lab 3 and 4 Objectives

Lab 3 and 4 Handout

Lab 3 and 4 PowerPoint

  

2/16, 2/18, 2/23 and 2/25

Lab 4

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Gram Stain

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Acid Fast Stain

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Spore Stain

 
 

See Above

 

2/25 Catch up, and review for practical day.    

3/2

 
Practical 1 Covers Labs 1-4    

Review for practical

3/4

 
Skills Test covers Smear making, staining, aseptic technique, use of scope and streak plating   Instructions and grading for skills test
3/6 Midterm:  Last day to drop without academic penalty (W)    
3/9 - 3/15 Spring Break, No classes    
3/16 and 3/18

Lab 5

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Bacterial Enumeration

 
 

 

 

 

Lab 5 Objectives

Power Point

Lab 5 background

Lab 5 protocol

How to use the spectrophotometer

Lab 5 sample calculations

 

3/23 and 3/25

Lab 6

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Oxygen Requirements

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Temperature Effects on Growth

 
  Lab 6 Objectives

Temperature handout

Oxygen handout

Lab 6 powerpoint

 

3/30 and 4/1

Lab 7

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Osmotic Pressure effects on Growth

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Lethal effects of temperature

 
 

 

Lab 7 Objectives

Lab 7 powerpoint

Lab 7 background and protocol

Lab 7 report sheet

4/6 and 4/8

Lab 8

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UV Light effects on Growth

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Antibiotic and antibacterial effects on growth
 

bullet Start lab 9 on April 8, swab nostrils to isolate organisms
  Lab 8 Objectives

Lab 8 antibiotics

Lab 8 UV

Lab 8 results/lab report

4/13, 4/15, 4/20 and 4/22

Lab 9:  Staphylococcus ID Lab

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4/13:  Isolate Staph and discuss project

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4/15: BAP and Slant

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4/20: Gram reaction, Rabbit plasma (coagulase), Slide Agglutination (staphyloside)

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4/22: Discussion of project, and Review for second practical

 

Review for lab practical 2

 

 

 

Staphylococcus lab handout

Staph ID sheet

Objectives 9

Lab 9 design/flow sheet

Agglutination test

 

Results

SA and SE on BAP

Staphyloslide test

Coagulase test

 

 

 

4/15 and 4/20

Lab 10: Case study and Diagnostic Microbiology

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4/15: Discuss case study, assign groups and inoculate media

 

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4/20: Discuss media, score media, work in groups

 

   
 
 

 

Objectives 10

Case Study

Media Powerpoint

Study Guide

 
4/27 Practical 2 Covers labs 5-10