Digestive System Physiology

This page last updated May 20, 2005

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Just FYI: Here's a picture of the results of a Benedict's test.  The tube on the left is showing a positive reaction, while the tube on the right shows a negative reaction.

 

This picture shows a positive starch reaction (left well) and a negative starch test (right well).

  
Review Questions:
  1. Why was pH a factor we measured in the lipase assay?
  2. You are testing an unknown enzyme and find that it functions best at a pH of 3, which part of the body is more likely to use this enzyme for digestion?
  3. Why did bile affect the activity of lipase?
  4. Why was BAPNA a good molecule to use for a pepsin assay?
  5. Why did we incubate the enzyme mixtures at 37 degrees Celsius?

Click here for answers to the questions.

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